[1]杨传凤,曹颖,胡尚连,等.基于慈竹转录组MYB基因的克隆及胁迫诱导表达[J].森林与环境学报,2015,35(01):60-66.[doi:DOI:10.13324/j.cnki.jfcf.2015.01.010]
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基于慈竹转录组MYB基因的克隆及胁迫诱导表达()
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《森林与环境学报》[ISSN:2096-0018/CN:35-1327/S]

卷:
35
期数:
2015年01期
页码:
60-66
栏目:
出版日期:
2015-01-15

文章信息/Info

文章编号:
2096-0018(2015)01-0060-07
作者:
杨传凤12 曹颖12 胡尚连12 徐刚12 黄艳12 龙治坚12
(1.西南科技大学植物细胞工程实验室,四川 绵阳 621010;2.四川省生物质资源利用与改性工程技术研究中心,四川 绵阳 621010)
关键词:
慈竹 MYB基因 克隆 生物信息学分析 诱导表达
分类号:
S718.46
DOI:
DOI:10.13324/j.cnki.jfcf.2015.01.010
文献标志码:
A
摘要:
从慈竹笋转录组数据库中克隆出2个MYB基因(BeMYB1、BeMYB2)进行生物信息学分析,探讨其响应脱落酸(ABA)、NaCl、聚乙二醇6000(PEG 6000)胁迫的机制。结果表明,BeMYB1与BeMYB2基因分别编码632个和338个氨基酸;BeMYB1蛋白属于MYB相关蛋白,与小麦TaMYB48蛋白聚为一枝,具有2个序列模体(motif);BeMYB2蛋白属于R2R3-MYB蛋白,与毛竹PeMYB2蛋白、水稻OsMYB18蛋白聚为一枝,具有3个motif。BeMYB1和BeMYB2基因均响应了ABA、NaCl和PEG 6000,但BeMYB2基因对胁迫的响应能力更强,BeMYB1和BeMYB2基因在响应非生物胁迫时发挥重要作用。

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备注/Memo

备注/Memo:
国家自然科学基金项目 (31400257 31400333) ;四川省应用基础研究基金项目 (2013JY0182) ;四川省生物质资源利用与改性工程技术研究中心基金项目 (12zxsk07 13zxsk01) ;西南科技大学研究生创新基金项目 (14ycxjj0079)
更新日期/Last Update: 2015-05-13