[1]张华峰.茉莉酸甲酯诱导巨桉对桉树枝瘿姬小蜂的抗性[J].森林与环境学报,2016,36(02):216-220.[doi:10.13324/j.cnki.jfcf.2016.02.014]
 ZHANG Huafeng.Effect of exogenous methyl jasmonate on induced resistance of Eucalyptus grandis seedlings against Leptocybe invasa[J].,2016,36(02):216-220.[doi:10.13324/j.cnki.jfcf.2016.02.014]
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茉莉酸甲酯诱导巨桉对桉树枝瘿姬小蜂的抗性()
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《森林与环境学报》[ISSN:2096-0018/CN:35-1327/S]

卷:
36
期数:
2016年02期
页码:
216-220
栏目:
出版日期:
2016-04-15

文章信息/Info

Title:
Effect of exogenous methyl jasmonate on induced resistance of Eucalyptus grandis seedlings against Leptocybe invasa
作者:
张华峰12
1. 厦门市绿化管理中心, 福建厦门 361004;
2. 福建农林大学林学院, 福建福州 350002
Author(s):
ZHANG Huafeng12
1. Xiamen Administration Department of Afforestation, Xiamen, Fujian 361004, China;
2. College of Forestry, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China
关键词:
茉莉酸甲酯桉树枝瘿姬小蜂诱导抗性防御酶
Keywords:
methyl jasmonateLeptocybe invasa Fisher et La Salleinduced resistancedefense enzymes
分类号:
S763
DOI:
10.13324/j.cnki.jfcf.2016.02.014
摘要:
为探讨茉莉酸甲酯(MeJA)诱导巨桉幼苗防御反应的生化机制及其对桉树枝瘿姬小蜂的抗性效应,在室内采用喷施不同浓度MeJA处理,测定并分析了MeJA对桉叶苯丙氨酸解氨酶(PAL)、超氧化物歧化酶(SOD)、过氧化物酶(POD)和多酚氧化酶(PPO)4种防御酶活性,以及胰蛋白酶抑制剂(TI)和胰凝乳蛋白酶抑制剂(CI)2种植物蛋白酶抑制剂活性的影响。结果表明,喷施MeJA处理24-148 h后,巨桉幼苗叶片PAL、SOD、POD和PPO四种防御酶和TI、CI两种蛋白酶抑制剂活性均有不同程度的变化;用1.00 mmol·L-1 MeJA处理后桉树枝瘿姬小蜂种群趋势指数为24.13,防治效果达70.98%。MeJA处理可诱导巨桉幼苗产生明显的防御反应,相关防御酶活性的变化与MeJA浓度和处理时间有关;MeJA可诱导巨桉幼苗产生抗虫性,对桉树枝瘿姬小蜂繁殖力和种群趋势指数产生影响,具有明显的防治效果。
Abstract:
In order to explicate the effect of exogenous methyl jasmonate (MeJA) on activities of related defense enzyme and induced resistance of Eucalyptus grandis seedlings against Leptocybe invasa Fisher et La Salle, effects on the activities of related defense enzymes and protease inhibitors such as phenylalanine ammonialyase (PAL), superoxide dismutase (SOD), peroxidase (POD), polyphemol oxidase (PPO), trypsin inhibitors (TI) and chymotrypsin inhibitors (CI) were analyzed by treating with MeJA at different concentrations. The effects of MeJA on the index of population trend and control effect of L. invasa were also measured. The results showed that after 24-148 h, the activities of PAL, SOD, POD, PPO, TI and CI changed in varying degrees. MeJA at 1.00 mmol·L-1 had obvious control effect of L. invasa with 70.98% and the index of population trend reached 24.13. These results indicated that MeJA induced E. grandis resistance against L. invasa which related to the increased activities of defense enzymes and protease inhibitors caused by MeJA.

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相似文献/References:

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[2]陈顺立,方晓敏,张思禄,等.不同抗虫品系桉树叶片背面组织的电镜观察[J].森林与环境学报,2014,34(04):322.
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 [J].,2012,32(02):345.
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备注/Memo

备注/Memo:
收稿日期:2015-09-23;改回日期:2015-11-19。
基金项目:福建省科技厅重点项目(2012N0008);福建省林业厅科技项目(闽林科函5号);福建农林大学科研基金项目(KF2015038)。
作者简介:张华峰(1977-),男,工程师,博士,从事林业有害生物综合防控管理和研究。
更新日期/Last Update: 1900-01-01