[1]王宁,袁美丽,安瑞云.低温对樟树抗寒性生理指标的影响[J].森林与环境学报,2020,40(05):512-518.[doi:10.13324/j.cnki.jfcf.2020.05.008]
 WANG Ning,YUAN Meili,AN Ruiyun.Effects of low temperature on the cold resistance physiology index of camphor tree[J].,2020,40(05):512-518.[doi:10.13324/j.cnki.jfcf.2020.05.008]
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低温对樟树抗寒性生理指标的影响()
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《森林与环境学报》[ISSN:2096-0018/CN:35-1327/S]

卷:
40
期数:
2020年05期
页码:
512-518
栏目:
出版日期:
2020-09-15

文章信息/Info

Title:
Effects of low temperature on the cold resistance physiology index of camphor tree
作者:
王宁1 袁美丽2 安瑞云2
1. 河南科技大学林学院, 河南 洛阳 471023;
2. 洛阳市隋唐城遗址植物园, 河南 洛阳 471002
Author(s):
WANG Ning1 YUAN Meili2 AN Ruiyun2
1. College of Forestry, Henan University of Science and Technology, Luoyang, Henan 471000, China;
2. The Sui and Tang Dynasties Relics Botanic Garden of Luoyang, Luoyang, Henan 471002, China
关键词:
叶绿素荧光抗氧化酶活性樟树低温胁迫
Keywords:
chlorophyll fluorescenceantioxidant enzyme activitycamphor treelow temperature stress
分类号:
S435.72
DOI:
10.13324/j.cnki.jfcf.2020.05.008
摘要:
以油樟[Cinnamomum camphora(terpineol-type)]和芳樟[C.camphora(linalool-type)]的1年生实生苗为研究对象,设置不同低温(0、6、12℃)及处理时间(0、2、4、6 d),探讨叶绿素含量、叶绿素荧光参数以及SOD活性等生理生化指标变化,并利用模糊隶属函数对其抗寒性进行评价,为耐寒樟树筛选提供参考。结果表明:不同低温条件下,2种樟树叶片内脯氨酸和MDA含量、SOD活性、相对电导率均随胁迫时间的延长呈持续增加的趋势,而叶绿素含量及最大光化学效率(Fv/Fm)则呈逐渐下降的趋势。此外,12℃条件下,2种樟树的初始荧光(F0)呈先降后升的趋势;而6℃和0℃条件下,均持续上升。0 ℃条件下至第6天时,油樟的SOD活性较CK增加显著(P<0.05),而芳樟的叶绿素和MDA含量则较CK下降显著(P<0.05)。相同低温条件下,随胁迫时间的延长,油樟叶绿素含量和Fv/Fm的降幅,以及MDA含量、相对电导率和F0的增幅均小于芳樟,而脯氨酸含量及SOD活性的增幅则大于芳樟。模糊隶属函数评价结果表明,油樟低温胁迫的适应性大于芳樟。
Abstract:
Using Cinnamomum camphora (terpineol-type) and C. camphora (linalool-type) as experimental materials, the dynamic variation of physiological and biochemical indexes such as chlorophyll content, SOD activity, and chlorophyll fluorescence parameters were investigated under conditions of 0, 6, 12 ℃ over 0, 2, 4, and 6 d; moreover, the cold resistance of the two camphor tree species was also analyzed. The results showed that: the content of proline and MDA, SOD activity, and relative conductivity of the two camphor species continuously increased, but the chlorophyll content and Fv/Fm decreased with the prolonged low-temperature treating time. The F0 of two camphor species decreased first but increased later at 12 ℃, but the F0 showed a continuous increase at 6 ℃ and 0 ℃. In addition, the SOD activity of C. camphora (terpineol-type) increased significantly compared to that of CK (P<0.05), while the contents of chlorophyll and MDA of C. camphora (linalool-type) decreased significantly compared to those of CK (P<0.05). During the different low-temperature stress conditions, the amplitude reduction of chlorophyll content and Fv/Fm, amplitude increase of MDA content, and relative electrical conductivity and F0 of C. camphora (terpineol-type) were lower than those of C. camphora (linalool-type). However, the amplitude increase of the proline content and SOD activity was greater than that of C. camphora (linalool-type). Finally, the cold resistance of two camphor tree species was evaluated as C. camphora (terpineol-type) > C. camphora (linalool-type) using the fuzzy mathematics method.

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备注/Memo

备注/Memo:
收稿日期:2020-06-17;改回日期:2020-08-03。
基金项目:河南省自然科学基金项目(182300410092)。
作者简介:王宁(1979-),男,讲师,博士,从事园林植物逆境生理研究。Email:hnkjdxwn@163.com。
更新日期/Last Update: 1900-01-01