[1]史世京,胡尚连,曹颖,等.冷冻胁迫下方竹抗氧化酶活性和叶绿素荧光特性[J].福建林学院学报,2013,33(01):38-42.
 SHI Shi-jing,HU Shang-lian,CAO Ying,et al.Preliminary studies on antioxidant enzyme activity and chlorophyll fluorescence parameters in Chimonobambusa quadrangularis under the condition of chilling and freezing stress[J].Journal of Fujian College of Forestry,2013,33(01):38-42.
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冷冻胁迫下方竹抗氧化酶活性和叶绿素荧光特性()
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《福建林学院学报》[ISSN:2096-0018/CN:35-1327/S]

卷:
33
期数:
2013年01期
页码:
38-42
栏目:
出版日期:
2013-01-15

文章信息/Info

Title:
Preliminary studies on antioxidant enzyme activity and chlorophyll  fluorescence parameters
in Chimonobambusa quadrangularis under the condition of chilling and freezing stress
文章编号:
1001-389X(2013)01-0038-05
作者:
史世京 胡尚连 曹颖 李想 卢学琴
(西南科技大学生命科学与工程学院,四川 绵阳 621010)
Author(s):
SHI Shi-jing HU Shang-lian CAO Ying LI Xiang LU Xue-qin
(Life Science and Engineering College, Southwest University of Science and Technology, Mianyang, Sichuan 621010, China)
关键词:
方竹 冷冻胁迫 抗氧化酶 叶绿素荧光参数
Keywords:
Chimonobambusa quadrangularis chilling and freezing stress antioxidant enzyme chlorophyll fluorescence parameters
分类号:
S795
文献标志码:
A
摘要:
以1年生盆栽实生方竹为材料,人工模拟冷害(4 ℃)、冻害(-5,-10,-20 ℃)和短时间(15,30,60 min)胁迫,测定抗氧化酶(CAT,POD,SOD)活性的变化,同时通过Imaging-PAM观测叶绿素荧光参数的变化,探讨方竹抵抗冷冻胁迫的机制。结果表明,冷害和冻害胁迫均可诱导方竹体内防御体系发挥作用,方竹叶片的相关抗氧化酶活性均表现出先升高后降低再升高的趋势,其中CAT和POD活性对低温胁迫的应答较为迟缓,而SOD活性对冷害和冻害胁迫的反应更灵敏。在冷害与冻害胁迫下,方竹叶片的叶绿素荧光参数Fv/Fm、Y(Ⅱ)、qP、ETR均呈现先降低后升高的趋势;与4 ℃低温的冷害胁迫相比较,冻害胁迫对光合作用的损伤更严重,其中在-20 ℃处理下,ETR、Y(Ⅱ)、qP皆为0,表明此时方竹已失去光合能力。
Abstract:
Using one-year old seedlings of Chimonobambusa quadrangularis as experimental material, effects of chilling (4 ℃) and freezing (-5,-10,-20 ℃) stress in a short time (15, 30, 60 minutes) on antioxidant enzyme (CAT, POD and SOD) activities and chlorophyll fluorescence parameters via Imaging-PAM instrument were investigated and observed, in order to explore mechanisms responding to chilling and freezing stress in Chimonobambusa quadrangularis. The results showed that among these antioxidant enzymes, compared with the responses of CAT and POD induced by chilling stress, changes of SOD activities were more sensitive both to chilling and to freezing stress. Moreover, chlorophyll fluorescence parameters, including Fv/Fm, Y(Ⅱ), qP and ETR, displayed the trend of “low-high” both in chilling and freezing conditions. However, fluorescence parameters under the cold-stress condition had greater changed scale than those under the freezing stress. ETR, Y(Ⅱ) and qP under the stress of -20 ℃  were hardly detected, suggesting plants had almost lost photosynthetic capacity.

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相似文献/References:

[1]李想,史世京,曹颖,等.冷冻胁迫下毛竹叶绿素荧光参数变化及抗寒相关转录因子表达[J].福建林学院学报,2014,34(01):57.

备注/Memo

备注/Memo:
基金项目:四川省应用基础研究基金资助项目(05JY029-101);四川主要丛生竹定向培育技术基金资助项目(10zx1102)。
更新日期/Last Update: 2015-03-13