[1]陆芝,林先云,张晓阳,等.刨花楠溃疡病菌生物学特性及其防治药剂筛选[J].森林与环境学报,2019,39(04):438-443.[doi:10.13324/j.cnki.jfcf.2019.04.017]
 LU Zhi,LIN Xianyun,ZHANG Xiaoyang,et al.Biological characteristics of pathogen of Machilus pauhoi canker and the selection of fungicides[J].,2019,39(04):438-443.[doi:10.13324/j.cnki.jfcf.2019.04.017]
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刨花楠溃疡病菌生物学特性及其防治药剂筛选()
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《森林与环境学报》[ISSN:2096-0018/CN:35-1327/S]

卷:
39
期数:
2019年04期
页码:
438-443
栏目:
出版日期:
2019-07-02

文章信息/Info

Title:
Biological characteristics of pathogen of Machilus pauhoi canker and the selection of fungicides
作者:
陆芝1 林先云2 张晓阳1 江仲鹏1 宋漳1 冯丽贞1
1. 福建农林大学林学院, 福建 福州 350002;
2. 顺昌县林业局, 福建 顺昌 353200
Author(s):
LU Zhi1 LIN Xianyun2 ZHANG Xiaoyang1 JIANG Zhongpeng1 SONG Zhang1 FENG Lizhen1
1. College of Forestry, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China;
2. Shunchang County Forestry Bureau, Shunchang, Fujian 353200, China
关键词:
刨花楠溃疡病病原菌生物学特性毒力
Keywords:
Machilus pauhoi Kanehiracankeronpathogenbiological characteristicstoxicity
分类号:
Q945.8
DOI:
10.13324/j.cnki.jfcf.2019.04.017
摘要:
为明确福建顺昌县刨花楠溃疡病病原菌SC-8(Lasiodiplodia margaritacea)的生物学特性和各种类药剂对病原菌的毒力大小,采用十字交叉法研究了不同培养条件对该病菌菌丝生长的影响,并用菌丝生长速率法测定了12种杀菌剂对病原菌的毒力。结果显示其菌丝最适生长温度为30℃;最适生长pH值范围为5~6;致死温度为50℃处理10 min;最适菌落生长碳源为果糖,最适氮源为硝酸铵;PDA是最适合菌落生长的培养基,其次是PSA;不同光照条件对菌丝生长有影响,在24 h光照菌丝生长最快。12种杀菌剂对菌丝的毒力测定结果表明,氟硅唑、精甲·咯菌腈、多菌灵、戊唑醇和苯甲·咪鲜胺的毒力较高,EC50分别为0.01、0.14、0.25、0.59和0.79 mg·L-1,异菌脲、百菌清和代森锰锌对病原菌的毒力最低,EC50分别为112.47、118.04和1 239.88 mg·L-1
Abstract:
To determine the biological characteristics of stain SC-8 (Lasiodiplodia margaritacea) and the virulence of various kinds of chemical agents to the pathogenic fungus caused Machilus pauhoi Kanehira canker in Shunchang County of Fujian Province in China, the biological characteristics of this pathogen was analysed by using the crossing and the toxicity of 12 fungicides to Lasiodiplodia margaritacea was tested by the method of mycelia growth in inhibition. The results showed that the optimum temperature of pathogenic fungus was 30℃; the most suitable growth pH ranged from 5 to 6; the lethal temperature was at 50℃ for 10 min; the optimum carbon source and nitrogen source were fructose and ammonium nitrate; the most suitable media for the growth of pathogen colony was PDA, followed by PSA; different light treatment had significant effects on growth of mycelia and 24 h lighting condition was most suitable for growth of mycela. Toxicity test of 12 fungicides on L. margaritacea indicated that the virulence of flusilazole, fludioxonil, carbendazim, tebuconazole and Benzol Prochloraz was higher, and the EC50 was 0.01, 0.14, 0.25, 0.59 and 0.79 mg·L-1, respectively. Virulence of iprodione, chlorothalonil and mancozeb to pathogen was the lowest, and EC50 was 112.47, 118.04 and 1 239.88 mg·L-1, respectively.

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备注/Memo

备注/Memo:
收稿日期:2019-3-1;改回日期:2019-4-18。
基金项目:福建省财政厅资助项目(131912040-K8112014A)。
作者简介:陆芝(1992-),女,硕士研究生,从事森林病理学研究。Email:1251677895@qq.com。
通讯作者:冯丽贞(1965-),教授,硕士生导师,从事树木生理学研究。Email:fjflz@126.com。
更新日期/Last Update: 1900-01-01