[1]杨华,宋绪忠,王秀云.高温胁迫对鹿角杜鹃的生理及生长影响[J].森林与环境学报,2020,40(03):284-289.[doi:10.13324/j.cnki.jfcf.2020.03.008]
 YANG Hua,SONG Xuzhong,WANG Xiuyun.Physiological responses of Rhododendron latoucheae to high temperature stress[J].,2020,40(03):284-289.[doi:10.13324/j.cnki.jfcf.2020.03.008]
点击复制

高温胁迫对鹿角杜鹃的生理及生长影响()
分享到:

《森林与环境学报》[ISSN:2096-0018/CN:35-1327/S]

卷:
40
期数:
2020年03期
页码:
284-289
栏目:
出版日期:
2020-05-15

文章信息/Info

Title:
Physiological responses of Rhododendron latoucheae to high temperature stress
作者:
杨华 宋绪忠 王秀云
浙江省林业科学研究院, 浙江 杭州 310023
Author(s):
YANG Hua SONG Xuzhong WANG Xiuyun
Zhejiang Academy of Forestry, Hangzhou, Zhejiang 310023, China
关键词:
鹿角杜鹃高温胁迫相对电导率超氧化物歧化酶丙二醛光合参数
Keywords:
Rhododendron latoucheae Franch.high temperature stressrelative conductivitysuperoxide dismutasemalondialdehydephotosynthetic parameter
分类号:
S685.21
DOI:
10.13324/j.cnki.jfcf.2020.03.008
摘要:
通过人工模拟设置对照[昼14 h/夜10 h (25 ℃/21 ℃), CK]、轻度胁迫(32 ℃/25 ℃)、中度胁迫(38 ℃/28 ℃)和重度胁迫(42 ℃/31 ℃)4个处理,分析比较鹿角杜鹃在不同高温胁迫处理下,相对电导率、丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性、净光合速率(Pn)、蒸腾速率(Tr)、胞间CO2浓度(Ci)、气孔导度(Gs)、水分利用效率(WUE)、气孔限制值(Ls)及叶片形态等指标的变化趋势,评价其对高温胁迫的响应及耐高温能力。结果表明:在CK和轻度胁迫下,随处理时间的延长,SOD活性、PnTr逐渐升高,MDA含量则先增加后减少,WUE和Ls下降;在中度胁迫下,SOD保持高活性,MDA积累不明显,PnTr下降,而WUE和Ls升高,光合作用是气孔限制的;在重度胁迫下,处理5 d时,植株老叶变褐,处理10 d时,相对电导率、MDA含量明显升高,处理14 d时,TrLs降至最低,Pn和WUE几乎为零,光合作用是非气孔限制的。因此,在重度胁迫下,SOD活性无法进行自我调节抵抗胁迫,细胞膜受到明显伤害,处理5 d后,植株出现不良表现,最后慢慢死亡。但在连续中度胁迫下,鹿角杜鹃维持细胞内活性氧产生和清除的平衡,维护细胞膜稳定,生长正常,适合应用于我国东南及中南部省份城市绿化。
Abstract:
Rhododendron latoucheae Franch. is widely distributed in the mountainous areas of south China. To assess its landscaping potential, we studied the physiological responses of R. latoucheae to high temperature stress. Four temperature gradients were simulated: control[daytime 14 h/night 10 h (25 ℃/21 ℃), CK], mild stress (32 ℃/25 ℃), moderate stress (38 ℃/28 ℃), and severe stress (42 ℃/31 ℃). Under each treatment condition, changes in the physiological indexes and leaf morphology were analyzed and compared. The results were as follows. In the CK and mild stress groups, superoxide dismutase(SOD) activity, photosynthetic rate(Pn), and transpiration rate(Tr) increased; malondialdehyde(MDA)content initially increased and then decreased; and water use efficiency(WUE) and stomata limitation(Ls) decreased over time. Under moderate stress, SOD activity exhibited high activity, MDA content fluctuated slightly, Pn and Tr declined, WUE and Ls increased, and photosynthesis was restricted by the stomata. Under severe stress, the leaves appeared brown by day 5. On the 10th day, the relative conductivity and MDA content increased sharply. Tr and Ls declined to the lowest values of any of the groups. Pn and WUE declined gradually, almost reaching zero by day 14, which was attributed to non-stomatal restriction. Under this severe stress treatment, SOD activity could not carry out the necessary self-regulation to resist stress, and the cell membrane was significantly injured. After 5 days of these conditions, the plant showed poor performance across all indices and finally died slowly. In contrast, under the continuous high temperature environment of the moderate stress treatment, intracellular reactive oxygen species production and clearance were balanced; further, cell membrane stability and normal growth were maintained. We therefore conclude that R. latoucheae is a suitable plant for urban greening in the southeast and southern-central provinces in China.

参考文献/References:

[1] 李小玲,华智锐,鲁霞霞.茉莉酸甲酯对秦岭高山杜鹃耐热性的影响[J].贵州农业科学, 2016, 44(12):23-27.
[2] 周广,孙宝腾,张乐华,等.井冈山杜鹃叶片抗氧化系统对高温胁迫的响应[J].西北植物学报, 2010, 30(6):1 149-1 156.
[3] 郑宇,何天友,陈凌艳,等.高温胁迫下西洋杜鹃的生理响应及耐热性[J].福建林学院学报, 2012, 32(4):326-335.
[4] 杨华,宋绪忠.高温胁迫对马银花的生理指标影响[J].林业科技通讯, 2016(1): 3-6.
[5] 张乐华,孙宝腾,周广,等.高温胁迫下五种杜鹃花属植物的生理变化及其耐热性比较[J].广西植物, 2011,31(5) : 651-658.
[6] 浙江植物志编辑委员会.浙江植物志:第五卷[M].杭州:浙江科学技术出版社,1992:12.
[7] 中国科学院中国植物志编委会.中国植物志:第57卷,第二册[M].北京:科学出版社,1994:358.
[8] 冯国媚.中国杜鹃花:第一册[M].北京:科学出版社,1988:224.
[9] 杨丽娟,顾地周,王秋爽,等.鹿角杜鹃嫩叶离体培养和高效植株再生[J].东北林业大学学报, 2012, 40(12): 51-54.
[10] 王剑敏,沈烈英,赵广琦.中亚热带优势灌木根系对土壤抗剪切力的影响[J].南京林业大学学报(自然科学版), 2011,35(2):47-50.
[11] 高俊香,梅盛龙,鲁小珍,等.凤阳山自然保护区麂角杜鹃种群结构与分布[J].南京林业大学学报(自然科学版), 2009,33(2):35-38.
[12] 张乐华,王书胜,单文,等.基质、激素种类及其浓度对鹿角杜鹃扦插育苗的影响[J].林业科学,2014, 50(3):45-54.
[13] 李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000:134-137, 260-263.
[14] 邹琦.植物生理学实验指导[M].北京:中国农业出版社,2000:11-12,163-165.
[15] 王凯红,刘向平,张乐华,等. 5种杜鹃幼苗对高温胁迫的生理生化响应及耐热性综合评价[J].植物资源与环境学报,201l,20(3):29-35.
[16] 黄溦溦,张念念,胡庭兴,等.高温胁迫对不同种源希蒙得木叶片生理特性的影响[J].生态学报,201l, 31(23):7 047-7 055.
[17] 欧祖兰,曹福亮,郑军.高温胁迫下银杏形态及生理生化指标的变化[J].南京林业大学学报(自然科学版), 2008,32(3):31-34.
[18] 王凤兰,周厚高,黄子锋,等.麝香百合抗热性生理生化指标及综合评价初探[J].广西植物, 2008, 28(2):261-264.
[19] 吴姝漪,孟千万,宋希强,等.高温胁迫对华石斛幼苗的形态和生理参数的影响[J].热带作物学报, 2017,38(4): 646-651.
[20] 张路,张启翔.高温胁迫对灰岩皱叶报春生理指标的影响[J].西南农业学报, 2011,24(5):1 728-1 732.
[21] MOHAMMED A R,TARPLEY L.Effects of high night temperature and spikelet position on yield-related parameters of rice (Oryza sativa L.) plants[J].European Journal of Agronomy,2010(33):117-123.
[22] 莫荣海,丁贵杰,罗仙英,等.不同家系马尾松苗木对持续干旱的响应[J].森林与环境学报, 2018, 38(4):473-480.
[23] 郑宇,何天友,陈凌艳,等.高温胁迫对西洋杜鹃光合作用和叶绿素荧光动力学参数的影响[J].福建农林大学学报(自然科学版), 2012,41(6):608-615.
[24] 周小燕,罗剑宁,李金月,等.有棱丝瓜苗期耐热性及其对热胁迫的生理响应[J].热带亚热带植物学报,2017,25(4):357-369.
[25] 杨雨华,宗建伟,王健胜,等.高温胁迫对杜仲幼苗部分生理指标的影响[J].东北林业大学学报, 2015, 43(3):14-17.
[26] 李小玲,雒玲玲,华智锐.高温胁迫下高山杜鹃的生理生化响应[J].西北农业学报, 2018,27(2): 253-259.
[27] 王丽娟,欧静,钟登慧,等.高温胁迫对桃叶杜鹃幼苗生理生化指标的响应[J].湖北农业科学, 2014, 53(20):4 882-4 884.

备注/Memo

备注/Memo:
收稿日期:2020-02-25;改回日期:2020-03-19。
基金项目:浙江省科技厅院所专项(2020F1065-1);浙江省林木新品种选育重大科技专项(2016C02056-12);浙江省林业科研成果推广项目(2019B09)。
作者简介:杨华(1976-),女,副研究员,博士,从事植物遗传育种研究。
更新日期/Last Update: 1900-01-01