[1]梁宇鹏,赵江源,杨佩文,等.哀牢山森林环境对铁载体子囊菌分布的影响[J].森林与环境学报,2020,40(03):298-305.[doi:10.13324/j.cnki.jfcf.2020.03.010]
 LIANG Yupeng,ZHAO Jiangyuan,YANG Peiwen,et al.Influences of the Ailao Mountain forest environment on the distribution of siderophore-producing Ascomycetes[J].,2020,40(03):298-305.[doi:10.13324/j.cnki.jfcf.2020.03.010]
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哀牢山森林环境对铁载体子囊菌分布的影响()
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《森林与环境学报》[ISSN:2096-0018/CN:35-1327/S]

卷:
40
期数:
2020年03期
页码:
298-305
栏目:
出版日期:
2020-05-15

文章信息/Info

Title:
Influences of the Ailao Mountain forest environment on the distribution of
siderophore-producing Ascomycetes
作者:
梁宇鹏1 赵江源1 杨佩文2 何翔2 文孟良1 李铭刚1
1. 云南大学云南省微生物研究所, 云南 昆明 650091;
2. 云南省农业科学院农业环境资源研究所, 云南 昆明 650205
Author(s):
LIANG Yupeng1 ZHAO Jiangyuan1 YANG Peiwen2 HE Xiang2 WEN Mengliang1 LI Minggang1
1. Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, China;
2. Institute of Agricultural Environmental Resources, Yunnan Academy of Agricultural Sciences, Kunming, Yunnan 650205, China
关键词:
子囊菌活性菌株铁载体环境因子聚类分析哀牢山
Keywords:
Ascomycetesactive strainssiderophoreenvironmental factorscluster analysisAilao Mountain
分类号:
Q938.1
DOI:
10.13324/j.cnki.jfcf.2020.03.010
摘要:
为了收集并定向发掘铁载体功能微生物资源,对云南省哀牢山国家级自然保护区原始森林土壤中的产铁载体微生物进行纯培养筛选、分离和鉴定,结合土壤真菌内转录间隔区(ITS)扩增子高通量测序结果,锚定活性菌株在群落结构中的位置,并据此分析活性菌株与环境因子的关系。结果表明:通过纯培养,从哀牢山低海拔河谷灌木林及次生林区域和高海拔原始森林区域土壤中筛选、分离和鉴定出的产铁载体真菌活性菌株,均为子囊菌门微生物,分属曲霉属(Aspergillus)、青霉属(Penicillum)、绿僵菌属(Metarhizium)、木霉属(Trichoderma)、篮状菌属(Talaromyces)及白僵菌属(Beauveria)。这些微生物主要分离自高海拔原始森林区域,其中的土样大多具备高有机质含量、高含水量、低pH值及低铁含量的特性。结合ITS高通测序结果,以上活性真菌菌株均有对应的可操作分类单元(OTU)。活性菌株相关属OTU分布与环境因子的相关性分析结果显示,青霉属的分布与有机质含量呈显著正相关;曲霉属的分布与pH值呈显著正相关,而与海拔呈显著负相关关系,与土壤质量含水量有一定的负相关关系,木霉属及篮状菌属的分布与所有环境因子的相关性均不显著,白僵菌属、绿僵菌属的分布与pH值有一定正相关关系。原始森林环境对微生物具有选择性影响,其中的产铁载体子囊菌是一类值得深入挖掘的重要功能性资源,其在原始森林土壤矿物质利用和有机质形成的良性循环过程中可能发挥了重要作用。
Abstract:
To collect and directionally explore siderophore-producing microorganisms, the siderophore-producing microorganisms in the native forest soil of the Ailao Mountain were screened, isolated, and identified using the pure culture method. Combined with the high-throughput sequencing results of soil internal transcribed spacer (ITS) amplicons, the position of active strains in the community structure was anchored. The relationship between active strains and environmental factors was analyzed further. The results showed that all of the active siderophore-producing fungal strains screened and isolated from the Ailao Mountain valley and the original forest soils through pure culture were Ascomycetes, which belonged to Aspergillus, Penicillium, Metarhizium, Trichoderma, Talaromyces, and Beauveria. These microorganisms were mainly isolated from high altitude virgin forest areas, in which most of the soil samples had high organic matter content, high water content, low pH value, and low ferrous content. Combined with the ITS high-throughput sequencing results, the above active strains all had the corresponding operational taxonomic units (OTUs). The results of the OTUs-environmental factor analysis showed that the Penicillium strains were significantly positively correlated with organic matter content; Aspergillus strains were significantly positively correlated with pH value but had a significant negative correlation with altitude and soil water content; Trichoderma and Talaromyces strains had not significantly correlated with all environmental factors; the distribution of Beauveria and Metarhizium had certain positive correlation with pH value. Thus, it is proposed that the primitive forest environment has a selective impact on the microorganisms. The siderophore-producing Ascomycetes from the primitive forest are important functional resources worthy of further exploration and may play an important role in the circulation of soil minerals and organic matter formation.

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备注/Memo

备注/Memo:
收稿日期:2020-01-06;改回日期:2020-03-24。
基金项目:国家自然科学基金项目(31760019;31663600)。
作者简介:梁宇鹏(1993-),男,硕士研究生,从事微生物资源开发与利用研究。Email:lypynu2019@163.com。
通讯作者:李铭刚(1973-),男,副研究员,从事微生物资源开发与利用研究。Email:mgli727@126.com。
更新日期/Last Update: 1900-01-01