[1]张晓阳,吴松,王美鑫,等.福建省樟树溃疡病病原菌的分离与鉴定[J].森林与环境学报,2020,40(03):306-312.[doi:10.13324/j.cnki.jfcf.2020.03.011]
 ZHANG Xiaoyang,WU Song,WANG Meixin,et al.Isolation and identification of camphor tree canker disease pathogen in Fujian Province[J].,2020,40(03):306-312.[doi:10.13324/j.cnki.jfcf.2020.03.011]
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福建省樟树溃疡病病原菌的分离与鉴定()
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《森林与环境学报》[ISSN:2096-0018/CN:35-1327/S]

卷:
40
期数:
2020年03期
页码:
306-312
栏目:
出版日期:
2020-05-15

文章信息/Info

Title:
Isolation and identification of camphor tree canker disease pathogen in Fujian Province
作者:
张晓阳1 吴松1 王美鑫1 韩森汇1 宋漳1 陈全助2
1. 福建农林大学林学院, 福建 福州 350002;
2. 福建农林大学金山学院, 福建 福州 350002
Author(s):
ZHANG Xiaoyang1 WU Song1 WANG Meixin1 HAN Senhui1 SONG Zhang1 CHEN Quanzhu2
1. College of Forestry, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China;
2. Jinshan College, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China
关键词:
樟树溃疡病病原鉴定毛色二孢属新壳梭孢属致病性福建省
Keywords:
Cinnamomum camphoracanker diseasepathogen identificationLasiodiplodiaNeofusicoccumpathogenicityFujian Province
分类号:
S763.15
DOI:
10.13324/j.cnki.jfcf.2020.03.011
摘要:
为了明确福建省樟树溃疡病的病原菌种类,对福建省福州市仓山区、三明市明溪县、南平市建阳区和顺昌县4个地区采集的樟树枝干溃疡样本进行分离、纯化和致病性分析,将病原菌进行系统形态学观察,并结合代表菌株的核糖体基因内转录间隔区(ITS)、翻译延伸因子(EF-1α)、RNA聚合酶Ⅱ大亚基(RPB2)和β-微管蛋白(BT)基因序列进行同源性分析,构建进化树,最终确定病原菌种类。结果表明,在福建省不同地区内采集到的75份樟树溃疡样本,经过组织分离、纯化和致病性检测,得到43个病原菌株,根据观察病原菌的菌丝生长状态和孢子特征,并结合系统进化树的结果,得到小新壳梭孢(Neofusicoccum parvum)、可可毛色二孢(Lasiodiplodia theobromae)、假可可毛色二孢(Lasiodiplodia pseudotheobromae)和Lasiodiplodia iranensis 4种类型菌株。接种菌株后发病症状与野外发病症状相同,但致病程度不同,其中小新壳梭孢最强,可可毛色二孢次之,L. iranensis最弱。
Abstract:
This study aimed to identify the Cinnamomum camphora canker disease pathogen in Fujian Province. We isolated, purified, and analyzed the pathogenicity of canker samples from four districts including Fuzhou City, Mingxi County of Sanming City, Jianyang District, and Shunchang County of Nanping City to determine the pathogen. The morphological characteristics of the pathogens were combined with the ITS, EF-1α, RPB2, and BT gene sequences of the representative strains for amplification and sequencing. This allowed the construction of the evolutionary tree, and the pathogens were finally determined. Among 75 dry ulcer samples collected from the camphor trees in different regions of Fujian Province, 43 pathogenic strains were obtained after tissue isolation, purification, and pathogenicity testing. Based on the status of mycelium growth and spore characteristics of pathogenic fungi and the results of phylogenetic trees, four strains were obtained, namely: Neofusicoccum parvum, Lasiodiplodia theobromae, Lasiodiplodia pseudotheobromae, and Lasiodiplodia iranensis. The pathogenicity test results showed that N. parvum was the strongest pathogen, followed by L. theobromae, and L. iranensis was the weakest.

参考文献/References:

[1] 余建雄,廖胜凤.香樟育苗与造林技术措施探寻[J].农业与技术,2018,38(17):70-71.
[2] 钟永达,田晓娟,李彦强,等.材用樟树研究进展[J].江西科学,2017,35(6):859-863.
[3] 曾令海,连辉明,张谦,等.樟树资源及其开发利用[J].广东林业科技,2012,28(3):62-66.
[4] 龙永彬,赵丹阳,秦长生,等.广东省樟树主要病害分析[J].林业与环境科学,2017,33(3):53-56.
[5] 郑郁.福建省尤溪县野生香樟调查研究与保护利用[J].中国林副特产,2015(4):78-80.
[6] 杨鼎超,衷诚明,郭铧艳,等.我国樟树病害分布及防治研究进展[J].生物灾害科学,2018,41(3):176-183.
[7] 郭立中,邓先琼,韦石泉.樟树的一种新病害:樟树溃疡病病原菌鉴定[J].植物病理学报,1995,25(1):28.
[8] 邓先琼,郭立中.樟树溃疡病病原菌再鉴定[J].湖南林业科技,2005,32(1):39-40.
[9] 翟立峰,张美鑫,赵行,等.重庆樟树溃疡病病原菌的鉴定及序列分析[J].林业科学研究,2019,32(3):18-25.
[10] 赵桂华.耧斗大茎点霉菌引起的樟树溃疡病病原菌特性研究[J].西部林业科学,2009,38(2):1-5.
[11] 王明生,吴小芹,王焱,等.上海市樟树病害种类调查及病害特征[J].中国森林病虫,2011,30(2):24-28.
[12] 方中达.植病研究方法[M].北京:农业出版社,1979.
[13] 马迪.园林植物国槐溃疡病致病真菌的生物学特性和致病酶活性的研究[D].聊城:聊城大学,2018.
[14] CRUYWAGEN E M, SLIPPERS B, ROUX J, et al. Phylogenetic species recognition and hybridisation in Lasiodiplodia: a case study on species from baobabs[J]. Fungal Biology, 2017, 121(4): 420-436.
[15] SAKALIDIS M L, HARDY G E S J, BURGESS T I. Use of the Genealogical Sorting Index(GSI)to delineate species boundaries in the Neofusicoccum parvum-Neofusicoccum ribis species complex[J]. Molecular Phylogenetics and Evolution, 2011, 60(3): 333-344.
[16] CROUS P W, SLIPPERS B, WINGFIELD M J, et al. Phylogenetic lineages in the Botryosphaeriaceae[J]. Studies in Mycology, 2006, 55: 235-253.
[17] KIRK P M, CANNON P F, MINTER D W, et al. Dictionary of the fungi[M]. 10th ed. Trowbridge: Cromwell Press, 2008.
[18] 廖旺姣,邹东霞,黄乃秀,等.广西桉树溃疡病病原菌鉴定[J].西南农业学报,2016,29(8):1 884-1 888.
[19] 张宗侠.樱花、核桃枝干溃疡类病害病原的鉴定及木霉对其的抑菌作用[D].泰安:山东农业大学,2012.
[20] LI G Q, LIU F F, LI J Q, et al. Characterization of Botryosphaeria dothidea and Lasiodiplodia pseudotheobromae from English Walnut in China[J]. Journal of Phytopathology, 2016, 164(5): 348-353.
[21] GOLZAR H, BURGESS T I. Neofusicoccum parvum, a causal agent associated with cankers and decline of Norfolk Island pine in Australia[J]. Australasian Plant Pathology, 2011, 40(5): 484-489.

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备注/Memo

备注/Memo:
收稿日期:2020-01-11;改回日期:2020-03-08。
基金项目:福建省科技厅项目(K1518090A;K1518075A);福建农林大学科技创新专项(CXZX2017121;CXZX2017501)。
作者简介:张晓阳(1993-),女,硕士研究生,从事森林病理学研究。Email:397501131@qq.com。
通讯作者:宋漳(1963-),男,教授,从事森林病理学研究。Email:fjszsz@126.com。
更新日期/Last Update: 1900-01-01