SUN Huale,ZHAI Xiaoqiao,CAO Xibing,et al.Proteomic analysis of the diploid and autotetraploid of Paulownia fortunei[J].,2020,40(06):619-626.[doi:10.13324/j.cnki.jfcf.2020.06.008]





Proteomic analysis of the diploid and autotetraploid of Paulownia fortunei
孙华乐1 翟晓巧2 曹喜兵1 邓敏捷1 赵振利1 范国强1
1. 河南农业大学泡桐研究所, 河南 郑州 450002;
2. 河南省林业科学研究院, 河南 郑州 450008
SUN Huale1 ZHAI Xiaoqiao2 CAO Xibing1 DENG Minjie1 ZHAO Zhenli1 FAN Guoqiang1
1. Institute of Paulownia, Henan Agricultural University, Zhengzhou, Henan 450002, China;
2. Forestry Academy of Henan, Zhengzhou, Henan 450008, China
isobaric tags for relative and absolute quantitation(iTRAQ)tetraploidPaulownia fortuneiproteomics
In order to study the protein changes during tetraploid formation in Paulownia fortunei, we analyzed the proteomic changes in the leaves of tetraploid P. fortunei and its diploid parents. This was done with tetraploid tissue culture seedlings of P. fortunei using the method of isobaric tags for relative and absolute quantitation(iTRAQ), and mass spectrometry quantitative techniques. The proteins related to ploidy formation were screened by association with the transcriptome. We identified 696 types of proteins,of which 75 were differentially expressed proteins,and these differentially expressed proteins were enriched into 40 gene ontology(GO) terms such as vacuole, cytoplasmic, and abiotic stimulus-response. Kyoto encyclopedia of genes and genomes(KEGG) analysis showed that these proteins were mainly involved in 35 metabolic pathways including photosynthesis, plant-pathogen interaction, and biosynthesis of secondary metabolites. Moreover, 9 differentially expressed proteins and genes, related to ploidy, were identified using transcriptome and proteome correlation analysis, and 4 of them were annotated into the KEGG metabolic pathway. These were magnesium chelatase H subunit, V-type H+ transporter ATPase subunit A, fructose diphosphate aldolase, and cytochrome C oxidase subunit 5B.


[1] COMAI L.The advantages and disadvantages of being polyploid[J].Nature Reviews Genetics,2005,6(11):836-846.
[2] KRIKORIAN A D.Paulownia in China:cultivation and utilization[J].Economic Botany,1988,42(2):283.
[3] 范国强,曹艳春,赵振利,等.白花泡桐同源四倍体的诱导[J].林业科学,2007,43(4):31-35.
[4] 张晓申,翟晓巧,赵振利,等.不同种四倍体泡桐光合特性的研究[J].河南农业大学学报,2013,47(4):400-404.
[5] 张晓申,刘荣宁,范国强,等.四倍体泡桐对干旱胁迫的生理响应研究[J].河南农业大学学报,2013,47(5):543-547,551.
[6] QIAO J J,WANG J X,CHEN L,et al.Quantitative iTRAQ LC-MS/MS proteomics reveals metabolic responses to biofuel ethanol in Cyanobacterial Synechocystis sp. PCC 6803[J].Journal of Proteome Research,2012,11(11):5286-5300.
[7] WANG Z,LIU W S,FAN G Q,et al.Quantitative proteome-level analysis of Paulownia witches’ broom disease with methyl methane sulfonate assistance reveals diverse metabolic changes during the infection and recovery processes[J].PeerJ,2017,5:e3495.
[8] 苏亚春.甘蔗应答黑穗病菌侵染的转录组与蛋白组研究及抗性相关基因挖掘[D].福州:福建农林大学,2014.
[9] ZHANG X S,DENG M J,FAN G Q.Differential transcriptome analysis between Paulownia fortunei and its synthesized autopolyploid[J].International Journal of Molecular Sciences,2014,15(3):5079-5093.
[10] DONG Y P,DENG M J,ZHAO Z L,et al.Quantitative proteomic and transcriptomic study on autotetraploid Paulownia and its diploid parent reveal key metabolic processes associated with Paulownia Autotetraploidization[J].Frontiers in Plant Science,2016,7:892.
[11] CASNEUF T,BODT S D,RAES J,et al.Nonrandom divergence of gene expression following gene and genome duplications in the flowering plant Arabidopsis thaliana[J].Genome Biology,2006,7(2):R13.
[12] SONG X M,WANG J P,SUN P C,et al.Preferential gene retention increases the robustness of cold regulation in Brassicaceae and other plants after polyploidization[J].Horticulture Research,2020,7:20.
[13] GARCíA-GARCíA A L,GRAJAL-MARTíN M J,GONZáLEZ-RODRíGUEZ á M.Polyploidization enhances photoprotection in the first stages of Mangifera indica[J].Scientia Horticulturae,2020,264:109198.
[14] CHENG J L,YANG X H,XUE L F,et al.Polyploidization contributes to evolution of competitive ability:a long term common garden study on the invasive Solidago canadensis in China[J].Oikos,2020,129(5):700-713.
[15] DONG Y P,FAN G Q,DENG M J,et al.Genome-wide expression profiling of the transcriptomes of four Paulownia tomentosa accessions in response to drought[J].Genomics,2014,104(4):295-305.
[16] DONG Y P,FAN G Q,ZHAO Z L,et al.Transcriptome expression profiling in response to drought stress in Paulownia australis[J].International Journal of Molecular Sciences,2014,15(3):4583-4607.
[17] XU E K,FAN G Q,NIU S Y,et al.Transcriptome-wide profiling and expression analysis of diploid and autotetraploid Paulownia tomentosa×Paulownia fortunei under drought stress[J].PLoS One,2014,9(11):e113313.
[18] FAN G Q,WANG L M,DENG M J,et al.Changes in transcript related to osmosis and intracellular ion homeostasis in Paulownia tomentosa under salt stress[J].Frontiers in Plant Science,2016,7:384.
[19] DONG Y P,FAN G Q,ZHAO Z L,et al.Transcriptome-wide profiling and expression analysis of two accessions of Paulownia australis under salt stress[J].Tree Genetics & Genomes,2017,13(5):97.
[20] WANG Z,ZHAO Z L,FAN G Q,et al.A comparison of the transcriptomes between diploid and autotetraploid Paulownia fortunei under salt stress[J].Physiology and Molecular Biology of Plants,2019,25(1):1-11.
[21] FAN G Q,LI X Y,DENG M J,et al.Comparative analysis and identification of miRNAs and their target genes responsive to salt stress in diploid and tetraploid Paulownia fortunei seedlings[J].PLoS One,2016,11(2):e0149617.
[22] SANMARTIN M,PATERAKI I,CHATZOPOULOU F,et al.Differential expression of the ascorbate oxidase multigene family during fruit development and in response to stress[J].Planta,2007,225(4):873-885.
[23] 郭燕, 朱杰, 许自成, 等. 植物抗坏血酸氧化酶的研究进展[J]. 中国农学通报, 2008(3):196-199.
[24] AXELSSON E,LUNDQVIST J,SAWICKI A,et al.Recessiveness and dominance in barley mutants deficient in Mg-chelatase subunit D,an AAA protein involved in chlorophyll biosynthesis[J].The Plant Cell,2006,18(12):3606-3616.
[25] ZHANG H T,LI J J,YOO J H,et al.Rice Chlorina-1 and Chlorina-9 encode ChlD and ChlI subunits of Mg-chelatase,a key enzyme for chlorophyll synthesis and chloroplast development[J].Plant Molecular Biology,2006,62(3):325-337.
[26] PONTIER D,ALBRIEUX C,JOYARD J,et al.Knock-out of the magnesium protoporphyrin IX methyltransferase gene in Arabidopsis:effects on chloroplast development and on chloroplast-to-nucleus signaling[J].The Journal of Biological Chemistry,2007,282(4):2297-2304.
[27] PAPENBROCK J,PFüNDEL E,MOCK H P,et al.Decreased and increased expression of the subunit CHL I diminishes Mg chelatase activity and reduces chlorophyll synthesis in transgenic tobacco plants[J].The Plant Journal,2000,22(2):155-164.
[28] KARP N A,HUBER W,SADOWSKI P G,et al.Addressing accuracy and precision issues in iTRAQ quantitation[J].Molecular & Cellular Proteomics,2010,9(9):1885-1897.
[29] HA M,LU J,TIAN L,et al.Small RNAs serve as a genetic buffer against genomic shock in Arabidopsis interspecific hybrids and allopolyploids[J].Proceedings of the National Academy of Sciences of the United States of America,2009,106(42):17835-17840.


更新日期/Last Update: 1900-01-01