[1]孙华乐,翟晓巧,曹喜兵,等.白花泡桐二四倍体的蛋白质组差异分析[J].森林与环境学报,2020,40(06):619-626.[doi:10.13324/j.cnki.jfcf.2020.06.008]
 SUN Huale,ZHAI Xiaoqiao,CAO Xibing,et al.Proteomic analysis of the diploid and autotetraploid of Paulownia fortunei[J].,2020,40(06):619-626.[doi:10.13324/j.cnki.jfcf.2020.06.008]
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白花泡桐二四倍体的蛋白质组差异分析()
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《森林与环境学报》[ISSN:2096-0018/CN:35-1327/S]

卷:
40卷
期数:
2020年06期
页码:
619-626
栏目:
出版日期:
2020-11-15

文章信息/Info

Title:
Proteomic analysis of the diploid and autotetraploid of Paulownia fortunei
作者:
孙华乐1 翟晓巧2 曹喜兵1 邓敏捷1 赵振利1 范国强1
1. 河南农业大学泡桐研究所, 河南 郑州 450002;
2. 河南省林业科学研究院, 河南 郑州 450008
Author(s):
SUN Huale1 ZHAI Xiaoqiao2 CAO Xibing1 DENG Minjie1 ZHAO Zhenli1 FAN Guoqiang1
1. Institute of Paulownia, Henan Agricultural University, Zhengzhou, Henan 450002, China;
2. Forestry Academy of Henan, Zhengzhou, Henan 450008, China
关键词:
同位素标记相对定量和绝对定量四倍体白花泡桐蛋白质组学
Keywords:
isobaric tags for relative and absolute quantitation(iTRAQ)tetraploidPaulownia fortuneiproteomics
分类号:
S763.1
DOI:
10.13324/j.cnki.jfcf.2020.06.008
摘要:
为研究二倍体白花泡桐在四倍体形成过程中蛋白质的变化,利用同位素标记相对定量和绝对定量(iTRAQ)以及液相串联质谱技术,分析了四倍体泡桐及其二倍体亲本叶片中的蛋白质表达变化,并通过与转录组的关联,筛选与四倍体形成相关的蛋白质。研究结果共鉴定到696个蛋白质,其中,有75个差异表达,对这些差异蛋白质进行基因本体论(GO)功能分析,其主要富集在液泡、胞浆和非生物刺激响应等40个基因本论条目中。对这些差异蛋白质进行京都基因与基因组百科全书(KEGG)分析表明,其主要参与35个代谢通路,包括光合作用、植物病原相互作用和次生代谢产物的生物合成等。通过蛋白质组和转录组关联分析,筛选到9个与倍性相关的差异蛋白质,基中4个注释到KEGG代谢通路,分别是镁螯合酶H亚基、V型H+转运ATPase亚基A、果糖二磷酸醛缩酶和细胞色素C氧化酶亚基5b。
Abstract:
In order to study the protein changes during tetraploid formation in Paulownia fortunei, we analyzed the proteomic changes in the leaves of tetraploid P. fortunei and its diploid parents. This was done with tetraploid tissue culture seedlings of P. fortunei using the method of isobaric tags for relative and absolute quantitation(iTRAQ), and mass spectrometry quantitative techniques. The proteins related to ploidy formation were screened by association with the transcriptome. We identified 696 types of proteins,of which 75 were differentially expressed proteins,and these differentially expressed proteins were enriched into 40 gene ontology(GO) terms such as vacuole, cytoplasmic, and abiotic stimulus-response. Kyoto encyclopedia of genes and genomes(KEGG) analysis showed that these proteins were mainly involved in 35 metabolic pathways including photosynthesis, plant-pathogen interaction, and biosynthesis of secondary metabolites. Moreover, 9 differentially expressed proteins and genes, related to ploidy, were identified using transcriptome and proteome correlation analysis, and 4 of them were annotated into the KEGG metabolic pathway. These were magnesium chelatase H subunit, V-type H+ transporter ATPase subunit A, fructose diphosphate aldolase, and cytochrome C oxidase subunit 5B.

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备注/Memo

备注/Memo:
收稿日期:2020-08-03;改回日期:2020-09-23。
基金项目:河南省林木种质资源保护与良种选育重点实验室开放课题(2020JB02008)。
作者简介:孙华乐(1991-),女,硕士研究生,从事林木生物技术研究。Email:shle0807@163.com。
通讯作者:范国强(1964-),男,教授,博士,从事泡桐丰产栽培理论与生物技术研究。Email:zlxx64@126.com。
更新日期/Last Update: 1900-01-01